This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union .

This Regulation shall be binding in its entirety and directly applicable in all Member States.

Done at Brussels, 23 September 2024.

For the Commission

The President

Ursula VON DER LEYEN

( 1 ) OJ L 396, 30.12.2006, p. 1 , ELI: http://data.europa.eu/eli/reg/2006/1907/oj

( 2 ) Commission Regulation (EC) No 440/2008 of 30 May 2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) ( OJ L 142, 31.5.2008, p. 1 , ELI: http://data.europa.eu/eli/reg/2008/440/oj ).

( 3 ) Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes ( OJ L 276, 20.10.2010, p. 33 , ELI: http://data.europa.eu/eli/dir/2010/63/oj ).

( 4 ) OECD Test Guideline 126: Determination of the Hydrophobicity Index of Nanomaterials Through an Affinity Measurement (2023), https://doi.org/10.1787/ae9c0fd1-en .

( 5 ) OECD Test Guideline 444A: In Vitro Immunotoxicity: IL-2 Luc Assay (2023) https://doi.org/10.1787/27b10ba3-en ; OECD Test Guideline 442C: In Chemico Skin Sensitisation: Assays addressing the Adverse Outcome Pathway key event on covalent binding to proteins (2023) https://doi.org/10.1787/9789264229709-en ; OECD Test Guideline 442E: In Vitro Skin Sensitisation: In Vitro Skin Sensitisation assays addressing the Key Event on activation of dendritic cells on the Adverse Outcome Pathway for Skin Sensitisation (2023), https://doi.org/10.1787/9789264264359-en .

( 6 ) OECD Test Guideline No 240: Medaka Extended One Generation Reproduction Test (MEOGRT) (2023) https://doi.org/10.1787/9789264242258-en ; OECD Test Guideline 218: Sediment-Water Chironomid Toxicity Using Spiked Sediment (2023) https://doi.org/10.1787/9789264070264-en ; OECD Test Guideline 219: Sediment-Water Chironomid Toxicity Using Spiked Water (2023) https://doi.org/10.1787/9789264070288-en .

( 7 ) OECD Test Guideline 125: Nanomaterial Particle Size and Size Distribution of Nanomaterials (2023) https://doi.org/10.1787/af5f9bda-en .

( 8 ) OECD Test Guideline 316: Phototransformation of Chemicals in Water – Direct Photolysis (2023) https://doi.org/10.1787/9789264067585-en .

( 9 ) OECD Test Guideline 405: Acute Eye Irritation/Corrosion (2023 https://doi.org/10.1787/9789264185333-en .

( 10 ) OECD Test Guideline 437: Bovine Corneal Opacity and Permeability Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage (2023), https://doi.org/10.1787/9789264203846-en .

( 11 ) OECD Test Guideline 438: Isolated Chicken Eye Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage (2023) https://doi.org/10.1787/9789264203860-en .

( 12 ) OECD Test Guideline 456: H295R Steroidogenesis Assay (2023) https://doi.org/10.1787/9789264122642-en .

( 13 ) OECD Test Guideline 458: Stably Transfected Human Androgen Receptor Transcriptional Activation Assay for Detection of Androgenic Agonist and Antagonist Activity of Chemicals (2023) https://doi.org/10.1787/9789264264366-en .

( 14 ) OECD, Test Guideline 460: Fluorescein Leakage Test Method for Identifying Ocular Corrosives and Severe Irritants (2023) https://doi.org/10.1787/9789264185401-en .

( 15 ) OECD Test Guideline 487: In Vitro Mammalian Cell Micronucleus Test (2023) https://doi.org/10.1787/9789264264861-en .

( 16 ) OECD Test Guideline 491: Short Time Exposure In Vitro Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage (2023) https://doi.org/10.1787/9789264242432-en .

( 17 ) OECD Test Guideline 492: Reconstructed human Cornea-like Epithelium (RhCE) test method for identifying chemicals not requiring classification and labelling for eye irritation or serious eye damage (2023) https://doi.org/10.1787/9789264242548-en .

( 18 ) OECD Test Guideline 496: In vitro Macromolecular Test Method for Identifying Chemicals Inducing Serious Eye Damage and Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage (2023) https://doi.org/10.1787/970e5cd9-en .

( 19 ) OECD Test Guideline 497: Defined Approaches on Skin Sensitisation (2023) https://doi.org/10.1787/b92879a4-en .

( 20 ) OECD Test Guideline 498: In vitro Phototoxicity – Reconstructed Human Epidermis Phototoxicity test method (2023) https://doi.org/10.1787/7b2f9ea0-en .

( 21 ) Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures, amending and repealing Directives 67/548/EEC and 1999/45/EC, and amending Regulation (EC) No 1907/2006 ( OJ L 353, 31.12.2008, p. 1 , ELI: http://data.europa.eu/eli/reg/2008/1272/oj ).

( 22 ) Section 2.2.4.2. of Annex I, Part 2, of Regulation (EC) No 1272/2008.

( 23 ) Section 2.2.4.4. of Annex I, Part 2, of Regulation (EC) No 1272/2008.

( 24 ) Table 2.6.4 of Annex I, Part 2 of Regulation (EC) No 1272/2008.

( 25 ) Section 2.6.4.4. of Annex I, Part 2 of Regulation (EC) No 1272/2008.

( 26 ) Section 2.8.4.1. of Annex I, Part 2, of Regulation (EC) No 1272/2008.

( 27 ) Section 2.15.4.1. of Annex I, Part 2, of Regulation (EC) No 1272/2008.

( 28 ) Section 2.16.2.1. of Annex I, Part 2, of Regulation (EC) No 1272/2008.

( 29 ) Section 2.17.2.1., points (b) and (c), and Section 2.17.2.2 of Annex I, Part 2 of Regulation (EC) No 1272/2008 of the European Parliament and of the Council.

COMMISSION REGULATION (EU) 2024/2492 of 23 September 2024 amending Regulation (EC) No 440/2008 as regards the test methods, to adapt them to technical progress ANNEX